Homogeneous membrane integrity assay, a rapid fluorescent measure of the release of cytoplasmic lactate dehydrogenase, is suitable for automated systems including HTS
With fewer steps and short incubation times, Promega's new CytoTox-One homogeneous membrane integrity assay is a fast, reliable way to detect and measure cell damage.
The assay is highly amenable to automation because sample transfer steps have been eliminated.
Therefore cytotoxic effects are measured without separating cells from supernatant.
The CytoTox-One assay is a rapid, fluorescent measure of the release of cytoplasmic lactate dehydrogenase (LDH) - a classic marker of cytotoxicity - into culture medium through damaged cell membranes.
Release of LDH is measured using a ten-minute coupled enzymatic assay that results in the conversion of resazurin to resorufin, a fluorescent product measured using a standard 96 or 384 well fluorometer. Assay reagents can be added directly to culture wells containing both viable and damaged cells without the need for washing or any other preparative steps.
Few steps and a short ten-minute incubation period enable more samples to be processed in a shorter period of time.
The CytoTox-One reagent does not compromise the integrity of viable cells.
Incubations can be carried out for longer periods if desired and assays read at a later time, so giving researchers greater flexibility in the way that they manage their assay throughput.
The CytoTox-One assay as well as saving time for laboratories carrying out cytotoxicity, or other cell viability assays, also lowers costs by reducing the amount of plastic ware needed compared to traditional LDH assays. In addition to its innovative, reliable and easy-to-use assays, Promega can offer the complete integrated systems for HTS in terms of reagents, luminometers and protocols.
