Qiagen PCR cloning kits combine the high efficiency of UA hybridisation with robust Qiagen EZ competent cells for fast and simple cloning of PCR products
The cloning procedure is easy.
Simply mix the PCR product directly with the pDrive Cloning Vector and the ready-to-use Ligation Master Mix, incubate 30 minutes, and then add to the cells for transformation.
Transformed cells can be plated directly onto agar/amplicillin plates without a recovery incubation The complete cloning procedure - from PCR product to plated cells - takes only 40 minutes, making it much faster than TA-based, topoisomerase-mediated, and conventional sticky- and blunt-end cloning methods.
Qiagen PCR cloning cits provide higher cloning efficiency than TA-based cloning.
The pDrive Cloning Vector contains 3' U, which has higher specificity for the A overhang of PCR products generated by Taq and other non-proofreading DNA polymerases.
The pDrive Cloning Vector has a large number of unique restriction enzyme recognition sites, universal sequencing primer sites, and promoters for in vitro transcription.
The vector allows both ampicillin and kanamycin selection as well as blue/white screening of recombinant colonies.
In addition, the Ligation Master Mix is specifically designed to provide optimal hybridization conditions for high cloning efficiency.
Qiagen PCR cloning kits are available in two convenient formats: both with and without Qiagen EZ competent cells.
These cells are supplied in the Qiagen PCR Cloningplus kit as ready-to-use aliquots for single transformation reactions.
