Gene expression system comprises a dedicated workstation with capacity for unattended purification and reaction setup of 96 samples in less than 90 minutes
Qiagen announces the launch of the BioRobot gene expression system - all you need to set up successful real-time RT-PCR analysis from living cells and tissue lysates! The gene expression system comprises a dedicated workstation with capacity for unattended purification and reaction setup of 96 samples in less than 90 minutes, and the RT-PCR specialist pack.
The BioRobot gene expression system saves valuable time by fully automating sample preparation - from cultured cells or tissue lysates, to RT-PCR mixtures that are ready to use in any real-time quantitative assay.
The installation and training programs, worktable accessories, chemistries for stabilization and purification, QuantiTect one-step RT-PCR enzyme technology, and certified QIAsoft protocols included in the RT-PCR specialist pack assure a fast startup, defined budgeting, and continued success.
Qiagen guarantees users of the BioRobot gene expression system optimal results at high throughput through seamless co-development of chemistries, automation platforms, and the QIAsoft operating system.
Using RNeasy technology, BioRobot yields over 90% recoveries of RNA at high purity (A260/A280=1.9) and with very high well-to-well repeatability, allowing highly accurate quantification of low-abundance targets.
Quantitative real-time RT-PCR analysis of 96 identical cell-culture samples gives threshold cycle (CT) values with a CV of <3%.
Threshold cycle values correlate linearly with cell numbers from 10 to 105 cells per well, allowing precise analysis over a wide range of sample concentrations even in the most sensitive RT-PCR applications.
