Up to 15mg protein per column can be purified under native or denaturing conditions, providing enough highly pure protein for a comprehensive functional and structural analysis
Ni-NTA Superflow columns enable up to 24 large-scale 6xHis-tagged protein preparations to be performed in parallel on the BioRobot 3000 workstation.
The pre-packed columns are pre-charged with nickel and can also be used for manual, gravity-flow purification.
Using cleared lysates as starting material, up to 15mg protein per column can be purified under native or denaturing conditions.
For protein structure analyses by NMR or X-ray diffraction, a single Ni-NTA Superflow column can provide enough highly pure protein for a comprehensive functional and structural analysis.
6xHis-tagged proteins are purified using proven Ni-NTA affinity chromatography, which delivers highly pure proteins in a simple bind-wash-elute procedure.
Cleared lysates from up to one liter of bacterial culture are pipetted onto a Ni-NTA Superflow column and drawn through under a carefully controlled vacuum, allowing efficient binding of 6xHis-tagged proteins.
After two wash steps, highly pure 6xHis-tagged protein is eluted using 3 ml of elution buffer.
Qiagen offers automated solutions for each stage of functional genomics, proteomics, and protein structure determination projects.
Initial expression-clone screening can be performed using fast, fully automated Ni-NTA magnetic agarose bead protocols.
The Ni-NTA Superflow 96 BioRobot kit enables high-throughput purification of up to 2mg 6xHis-tagged protein per well, sufficient for initial functional studies.
