Resonance light scattering is based on the optical light-scattering properties of nano-sized metal colloid particles illuminated by a standard white light source
Qiagen HiLight dual-colour kits provide efficient dual-colour labelling and highly sensitive detection for a wide variety of arrays.
Compared with standard fluorescent dyes, the non-fluorescent HiLight system provides superior sensitivity just a tenth of the starting RNA.
This enables detection of more positive genes - without amplification - giving the true expression pattern of low-abundance genes.
Resonance light scattering (RLS) technology, used by the HiLight system, is based on the optical light-scattering properties of nano-sized metal colloid particles illuminated by a standard white light source. The intense signals produced by silver and gold HiLight RLS particles enable simple and highly sensitive detection of low-abundance mRNAs using just 1-2µg total RNA - without amplification.
Because of the metallic nature of the particles, there is no reduction of signal due to photobleaching.
Samples can be re-measured multiple times to detect low- and high-intensity signals, each with optimum exposure times, on the same microarray.
HiLight dual-colour kits provide optimised silver and gold HiLight RLS particles and hybridisation, washing, blocking, and staining reagents, with protocols to perform experiments on five or 24 separate microarray slides.
In addition, the HiLight dual-colour kit Plus comes with the LabelStar array kit for equal dual-colour labelling and efficient cleanup of cDNA.
This guarantees optimum labelling conditions for dual-colour HiLight detection.
Detection is performed on the easy affordable HiLight reader.
Compatible with arrays spotted on Corning Gaps slides, and MWG Pan and PerkinElmer Micromax microarrays.
For other arrays, please enquire.
