Myeloperoxidase (MPO) assay offers a rapid, reliable and simple tool for quantitation of neutrophil infiltration in mouse tissues
Assessment of tissue myeloperoxidase (MPO) content is widely used for quantitation of neutrophil infiltration.
The new Mouse MPO assay from Cambridge BioScience offers a rapid, reliable and simple tool for quantitation of neutrophil infiltration in mouse tissues.
The assay, developed by HyCult Biotechnology (HBT), is based on an Elisa immunoassay consisting of highly specific MPO antibodies.
This method is not influenced by tissue enzyme inhibitors and allows accurate and easy detection of MPO present in infiltrating neutrophils.
The Mouse MPO Assay uses a method devoid of the cumbersome blockade of inhibitory factors which is necessary in the classical enzymatic assay.
Organs, particularly the kidney and liver, contain large amounts of inhibitors that disturb the enzymatic MPO assay in such tissues.
Furthermore, the assay can be used for quantitation of MPO in plasma such as after LPS administration and during inflammation and in vitro release of MPO by neutrophils.
