Prepacked columns are designed to meet the growing need for pure proteins to use in functional and structural genomics and proteomics research
GE Healthcare has announced the availability of HisTrap FF crude (1ml and 5ml) prepacked columns designed to meet the growing need for pure proteins to use in functional and structural genomics and proteomics research.
These new columns enable fast, reliable and simple one-step purification of histidine-tagged recombinant proteins directly from unclarified cell lysates.
The HisTrap FF crude columns minimise the need for sample pre-treatment.
Sonicated cell lysate can be directly loaded onto the column, eliminating the need for a clarification step (centrifugation or filtration) and offering a time savings during the purification process.
The fast one-step purification provided by HisTrap FF crude columns is ideal for researchers working with proteins easily degraded by proteases in the cell lysate.
"The elimination of the pre-treatment stage offers scientists substantial time savings," said Allan Simpson, vice president of research and analysis, protein separations at GE Healthcare.
"The prepacked feature of the HisTrap FF crude columns ensures that customers with limited experience in protein purification will be able to achieve reliable reproducibility during purification of histidine-tagged proteins".
HisTrap FF crude columns are prepacked with Ni Sepharose 6 Fast Flow and can be used with any chromatography system, a laboratory pump, or a simple syringe.
The medium is compatible with a wide range of reducing agents, detergents and other additives.
In combination with negligible leakage of nickel ions, histidine-tagged protein purification is conveniently performed resulting in pure, active protein.
