High-speed preparation of cDNA from cultured cells without RNA purification makes it easy to perform real-time, two-step RT-PCR analysis of several samples within a few hours
With the FastLane cell cDNA kit, only 45 minutes are required to prepare first-strand cDNA directly from cultured cells - no RNA purification steps are necessary.
The high-speed procedure makes it easy to perform real-time, two-step RT-PCR analysis of several samples within a few hours.
The FastLane cell cDNA kit integrates rapid cell lysis, immediate RNA stabilisation, elimination of genomic DNA, and efficient and sensitive reverse transcription, says Qiagen.
The simple workflow makes the kit ideal for applications such as validation of siRNA-mediated gene knockdown or snapshot analysis of gene expression in cells under different conditions.
Highly reproducible and sensitive reverse transcription can be achieved, says the company.
Rapid cell lysis with simultaneous RNA stabilisation ensures RNA transcripts remain intact, while optimal reaction conditions for reverse transcription provide high cDNA yields.
Even difficult templates, such as those with high GC-content or complex secondary structure, are successfully reverse-transcribed.
To ensure only RNA is detected in real-time RT-PCR, the FastLane cell cDNA kit uses a novel buffer to effectively eliminate genomic DNA contamination.
Time and effort are saved as there is no need to design RNA-specific primers or probes, which may not even be possible with some genes.
