Protein enrichment kit reduces host proteins by 90% or more without compromising expression of the target protein to produce a much cleaner initial prep and facilitate downstream purification
The EpiGold protein enrichment kit, available from Cambridge BioScience, significantly lowers levels of endogenous E coli proteins during recombinant protein purification.
Developed by Genlantis, the EpiGold kit is said to reduce host proteins by 90% or more without compromising expression of the target protein.
This produces a much cleaner initial prep and facilitates significant improvements in downstream purification.
For untagged proteins, the EpiGold kit is guaranteed to produce at least a ten-fold enrichment of target protein expression.
The EpiGold system allows researchers to continue using their preferred expression vectors, coding sequences and purification tags.
A construct is simply transformed into the optimised EpiGold eBL21 E coli.
These cells are grown with the EpiGold complex to achieve excellent suppression of endogenous bacterial protein expression.
The EpiGold protein enrichment kit is effective for both soluble and insoluble proteins and provides comparable recombinant protein yields from EpiGold-treated cultures versus untreated cultures.
The EpiGold kit also works with tagged proteins, reducing any contaminating host proteins that co-elute with the target.
These products are available from Cambridge BioScience in the UK and Ireland only.
