Small interfering RNA (siRNA) can be ordered in either the standard 21mer or in the new 27mer format, which has recently attracted scientific interest as it is assumed to provide higher efficiency
Nucleic acids specialist MWG Biotech announces the extension of its siRNA portfolio (small interfering RNA - used for analysing the function of genes and for silencing gene activity), with the addition of multiple scales and siRNA in either the classical 21mer or the new 27mer format, plus a new advanced siRNA online design tool.
MWG offers licenced high quality custom siRNA and pre-designed siRNA synthesis, produced with proprietary siMax technology (an advanced TBDMS-based synthesis chemistry) which it says allows reliable synthesis, high coupling yields and greatly reduced interfering n-x products in fast turn-around time.
MWG Biotech sells the new MWG siRNA products under the siMax brand name.
MWG Biotech now offers three different scales, with improved yields on the already existing scale.
siRNA can be ordered in either the standard 21mer or in 27mer format.
This new format has recently attracted much scientific interest as it is assumed to provide a higher efficiency in comparison to the classical 21mer siRNA.
As an extra benefit, MWG Biotech offers the first commercial design program to view mRNA accessibility.
Customers can view the secondary structure of the siRNA/mRNA target site to select designs in regions of optimal secondary structure in order to improve silencing efficiency.
The company says its on-line design tool set gives customers access to the latest bioinformatics design algorithms.
siRNA candidate sequences can also undergo Blast analysis to reduce off-target effects.
All siMax siRNA molecules are delivered annealed, purified (desalted or HPLC-purified) and ready to use.
All products are normalised to ensure optimum duplex formation.
Each single strand of RNA is analysed by Maldi-Tof mass spectrometry and capillary gel electrophoresis (CGE).
Maldi-Tof mass spectrometry is used to confirm the sequence identity of the individual single strands.
Purity of the strands is then confirmed using CGE analysis.
Both Maldi-Tof and CGE traces of the single strands are provided free of charge.
