Cambridge BioScience is offering a series of fluorescent labelling dyes that span the full visible spectrum.
Cambridge BioScience is offering a series of fluorescent labelling dyes that span the full visible spectrum.
Manufactured by AnaSpec, a specialist in fluorescence resonance energy transfer (FRET) technologies, this product line has been developed to solve various limitations with existing fluorescent labelling reagents.
HiLyte Fluor dyes have better labelling performances than the classic fluorescent labelling dyes such as FITC and rhodamines, says Cambridge BioScience.
Furthermore, the conjugated dyes exhibit more intense fluorescence than other spectrally similar conjugates of classic fluorescent dyes, such as FITC, TAMRA and ROX, under similar excitations.
In addition to being more photo-stable, HiLyte Fluor dyes are highly fluorescent over a broad pH range with little pH sensitivity.
The dyes and their conjugates are available in several distinct fluorescent colours.
They have absorption spectra that match the principal output wavelengths of common excitation sources including 488nm, 555nm, 633nm and 647nm.
Most of the HiLyte Fluor dyes have good water solubility, so protein conjugations can be performed without organic solvents, and the conjugates are resistant to precipitation during storage.