Complete whole genome amplification and reamplification kits are derived from proprietary amplification method based on random fragmentation of the genome into a series of overlapping short templates
Sigma-Aldrich announces two additions to the GenomePlex whole genome amplification (WGA) family of products: the GenomePlex complete whole genome amplification kit and the GenomePlex WGA reamplification kit.
Many researchers today performing whole genome amplification assays have a need for increased amplification accuracy in addition to finding a method that preserves precious source material.
To address these needs, Sigma-Aldrich provides options with the GenomePlex complete whole genome amplification kit (catalogue number WGA2) and the GenomePlex WGA reamplification kit (catalogue number WGA3).
The GenomePlex complete WGA kit (WGA2) contains the same reagents as the original GenomePlex WGA kit (WGA1), as well as an optimised enzyme, WGA DNA polymerase.
This enzyme provides for increased accuracy in amplification by producing no amplicon in the negative control reactions.
Assays using the GenomePlex complete WGA kit provide results showing complete representation of the entire genome with no detectable allele bias.
This product is now available to purchase.
The third member to the product line, GenomePlex WGA reamplification kit, allows subsequent reamplifications of the initial whole genome amplification product.
The successive reamplification process provides microgram quantities of DNA with little genetic bias when compared to the original genome.
The GenomePlex WGA reamplification kit contains the WGA DNA polymerase and the 10x master amplification mix.
The optimised enzyme, WGA DNA polymerase, decreases the background in the reactions and leads to more accurate results in downstream PCR or hybridisation techniques.
This product is expected to be available to purchase in October 2005.
"The GenomePlex complete WGA and GenomePlex WGA reamplification kits are excellent complements to Sigma's whole genome amplification product offering," said Keith Jolliff, director of global marketing, molecular biology at Sigma-Aldrich.
"The GenomePlex complete WGA kit resolves concerns of many researchers who see product in their negative control samples after running whole genome amplification assays.
"In addition, the successive reamplification process provided by the GenomePlex WGA reamplification kit gives researchers another option for preserving their precious source material in microgram quantities".
Both kits are derived from the proprietary amplification method that is based upon random fragmentation of the genome into a series of overlapping, short templates.
The resulting shorter DNA strands are efficiently primed and amplified to generate a library of DNA fragments with defined three primed and five primed termini, the OmniPlex library.
This library is replicated using a linear, isothermal amplification in the initial stages, followed by a limited round of geometric (PCR) amplifications.
The GenomePlex whole genome amplification (WGA) technology has been used in a variety of applications and is suitable for use with purified genomic DNA from a variety of sources including blood cards, whole blood, buccal swabs, soil, plant, and formalin-fixed paraffin-embedded (FFPE) tissues.
GenomePlex WGA uses nanogram quantities of starting genomic DNA, which after PCR yields 5-10ug of WGA product.
After purification, the WGA product can be analysed in a manner similar to any genomic or chromosomal DNA sample.
A number of downstream applications may be performed including TaqMan assays, CGH analysis, SNP analysis, sequencing, etc.
The GenomePlex whole genome amplification technology is licensed by Rubicon Genomics.
