Columns purify histidine-tagged proteins quickly and simply from clarified or unclarified samples without the need for a pump or purification system
GE Healthcare has announced the availability of His GraviTrap 1ml prepacked columns to meet the ever-growing need for pure proteins to use in functional and structural genomics and proteomics research.
His GraviTrap columns purify histidine-tagged proteins quickly and simply from clarified or unclarified samples without the need for a pump or purification system.
Their protein binding capacity (approx 40mg/column) is among the highest available today, says GE Healthcare.
Separations are completed in as little as 20 minutes, large sample volumes can easily be applied, and concentrated purified protein elutes in a small volume.
"Short purification times and simple, gravity-flow operation are performance benefits that will be appreciated by all scientists needing to run reliable, time-saving protein purifications," said Allan Simpson, vice president of research and analysis, protein separations at GE Healthcare.
"In addition, new accessories further increase speed and convenience.
"For example, the His Buffer Kit reduces preparation time and optimises results, with the LabMate reservoir enabling sample volumes up to 35ml to be loaded in one run".
His GraviTrap columns are prepacked with Ni Sepharose 6 Fast Flow.
Purifications can be performed under either native or denaturing conditions using a wide range of detergents, reducing agents and other additives.
Nickel ion leakage is negligible, which minimises protein precipitation and increases the yield of pure and active material.
