Pipette tips contain the unique 'zwitterionic' Zic-hilic chromatographic particle for extraction and purification of hydrophilic biomolecules such as glycopeptides.
SeQuant announces the availability of Zic-tip, pipette tips which contain the unique 'zwitterionic' Zic-hilic chromatographic particle for extraction and purification of hydrophilic biomolecules such as glycopeptides.
A typical application of Zic-hilic tips is the selective extraction of glycopeptides from more abundant, less hydrophilic peptides; this greatly facilitates their subsequent identification with LC/MS or Maldi.
Extraction can be readily combined with techniques for partial deglycosylation to provide a powerful route to assignment of post-translational glycosylation sites in a protein.
Zic-tip pipette tips are available in two different formats.
The Zic-tip mini contains a packed bed of chromatographic particles and is used as a miniature solid phase extraction (SPE) device.
Sample loading, washing and elution are accomplished by adding the appropriate liquid on the top of the chromatographic bed.
The tips can be used as spin-columns by placing them in centrifuge tubes using the supplied adapters.
Zic-tip mini is suitable for sample volumes of about 2-200ul.
The Zic-tip micro pipette tips have chromatographic material attached to the wall of the tip.
This allows the sample, washing and extraction solutions to be repeatedly aspirated and dispensed easily with no flow resistance through the pipette tip for enhanced extraction and purification efficiency.
Zic-tip micro is suitable for extraction volumes of about 0.1-100ul.
Zic-tip pipette tips are completely free from frits, filters and glue, says the company, ensuring minimum flow resistance, minimum sample loss and absolutely no sample contamination.
They are available in 10ul and 200ul tip volumes.
