The production of antibodies against phosphopeptides has often presented a challenge for researchers in search of high-quality results
Much of the challenge has been attributed to the finicky stability of the phosphorylation on the peptide.
AnaSpec, a provider of integrated proteomics solutions, believes that its expertise in synthesizing phosphorylated peptides will be crucial in answering the need for high-quality phosphospecific antibodies.
As a result, AnaSpec now offers custom phosphospecific antibody production as one of its major antibody services.
AnaSpec offers a complete Phosphopeptide Antibody Package that includes: 15mg (90% purity, up to 15 amino acids) phosphospecific peptide - 5 mg delivered to researcher, 10 mg for antibody production.
11mg (80% purity, up to 15 amino acids) non-phosphospecific peptide - used for affinity depletion (in-house only).
Immunisation of two rabbits.
Phosphospecific and non-phosphospecific peptide Elisa titration of antibody for all bleeds.
Pre-immune sample of serum (5ml/each rabbit) and anti-serum (100ml total).
Affinity purification of selected batch of serum.
Shipment of purified phosphospecific antibody which does not cross-react with non-phosphospecific peptides (as confirmed by Elisa).
