Introduction to HPLC pumps and eluent gradients

This interactive tutorial is available online as well as on CD.

The authors are John Dolan, who writes the HPLC Troubleshooting column in LCGC Magazine, and Dennis Saunders, the creator of previous versions of Savant's series of computer-based HPLC training programmes.

In HPLC, the composition and flow of eluent through the column is critical to a successful separation.

This module introduces the basic equipment and discusses solvent properties, eluent materials and additives, pumps, gradient elution and gradient formation, filters and injection equipment.

Building on the best features of the earlier versions, such as the use of interactive animations and simulations to explain and illustrate chromatographic concepts, the programme features a modernized interface, narration, an assessment quiz with automatic scoring and revision hyperlinks, a glossary and bibliography.

The customisation capability enables instructors to augment the basic content.