Seeking to secure its position as one of the top two providers of catalogue peptides in the world, this week AnaSpec introduced a selection of new peptides for drug discovery research
Bacterial peptides.
CFTR (108-117); Pseudomonas aeruginosa Inhibitor (Cat# 61810-1/61810-5).
Sequence: SYDPDNKEER.
This peptide represents amino acids 108-117 of the first CFTR (human cystic fibrosis transmembrane conductance regulator) extracellular domain.
This peptide has been shown to inhibit internalisation of Pseudomonas aeruginosa.
Protein phosphorylation related peptides.
Histone H3 (116 - 136); C116 - 136 (Cat# 61704).
Sequence: KRVTIMPKDIQLARRIRGERA.
This peptide spans the C-terminus of histone H3; amino acids 116 to 136.
Histone H3 (1-21); Biotinylated (Cat# 61702).
Sequence: ARTKQTARKSTGGKAPRKQLA-GG-K(BIOTIN)-NH2.
This sequence corresponds to the first 21 amino acids of the NH2 terminal of histone H3 followed by a GG linker and a biotinylated lysine.
This peptide was used to investigate the characteristics and mechanisms of ethanol-induced histone H3 acetylation in rat hepatocytes.
Immunocytochemical and immunoblot analyses revealed that ethanol treatment significantly increased H3 acetylation at Lys9 with negligible effects at Lys14; 18; and 23.
RS domain derived peptide (Cat# 61722).
Sequence: GRSRSRSRSR.
This peptide is a substrate for Clk/Sty and is phosphorylated by Clk/Sty protein kinase (Km=102 microM).
BTK derived peptide (Cat# 61728).
Sequence: KKVVALYDYMPMN-NH2.
BTK derived peptide is a substrate for Btk (Bruton's tyrosine kinase); a nonreceptor tyrosine kinase; involved in precursor B (pre-B) cell receptor signal which is required for normal B cell development and signal transduction through cell surface molecule.
Histone H3 (116 - 136); N15 - 39 (Cat# 61705).
Sequence: APRKQLATKAARKSAPATGGVKKPH.
This peptide is based on amino acids 15 to 39 of histone H3.
Forkhead derived peptide; FAM-labeled; Woodtide (Cat# 61744).
Sequence: 5-FAM-KKISGRLSPIMTEQ-NH2.
A FAM-labeled Forkhead derived peptide (Ab/Em = 494/521 nm).
Also known as woodtide; this peptide is used as a substrate for the DYRK family of kinases in in-vitro analysis.
The peptide corresponds to residues 324-334 of transcription factor FKHR with two lysine residues added at the N-terminus to facilitate binding to phosphocellulose paper.
Forkhead derived peptide; Woodtide (Cat# 61743).
Sequence: KKISGRLSPIMTEQ-NH2.
This peptide is used as a substrate for the DYRK family of kinases in in-vitro analysis.
Also known as woodtide; this peptide corresponds to residues 324 to 334 of transcription factor FKHR with two lysine residues added at the N-terminus to facilitate binding to phosphocellulose paper.
Viral Peptides.
LMP2 (340 - 350); SSC (Cat# 61681).
Sequence: SSCSSCPLSKI.
This is an Epstein-Barr virus (EBV)-encoded oncogene latent membrane protein 2 (LMP2); HLA-A11 restricted subdominant epitope.
The SSC-specific effector CTLs; active against both the peptide and endogenously expressed LMP2 protein; could be generated from donor (HLA-A11-positive) by stimulation with SSC-loaded dendritic cells(SSC/DC).
LMP2 (419-427); TYG (Cat# 61683).
Sequence: TYGPVFMCL.
This is a HLA A*2402-restricted Epstein-Barr virus (EBV)-encoded oncogene latent membrane protein 2 (LMP2) epitope fragment amino acid residues 419 to 427.
HIV QK10 (Cat# 61672).
Sequence: QVPLRMTYK.
This is a HLA A3 restricted HIV Nef epitope (QK10); a conserved CTL epitope within a functional domain of nef.
It overlaps with CTL epitopes restricted by HLA A11; B7 and B35.
Neuropeptides.
L1ARSLCD (Cat# 61661).
Sequence: FGEARSLESDNEE.
This peptide has a single amino acid substitution (Y1176A) at critical tyrosine residue compared to L1CD (1173-1185).
It corresponds to the mutant form of L1 amino acids 1173 to 1185.
The L1 subfamily of cell adhesion molecules (CAMs) has a highly conserved cytoplasmic domain that contains a tyrosine; followed by the alternatively spliced RSLE (Arg-Ser-Leu-Glu) sequence.
