Thermo Fisher Scientific has announced the availability of the Cellomics Synaptogenesis high content screening (HCS) reagent kit.
The kit is said to enable simultaneous detection of neuronal population, neurite, pre-synaptic vesicle, post-synaptic puncta and synapse using a fixed end-point assay that is based on immunofluorescence detection in cells grown on standard high-density microplates.
The Thermo Scientific Cellomics Synaptogenesis HCS reagent kit has been designed to enable drug discovery and systems biology researchers to measure synaptic function utilising the benefits of a new technology in high-content cell-based image analysis.
The kit is said to offer simplicity and streamlined preparation of quality samples in the laboratory.
It has been optimised with a Thermo Scientific Arrayscan HCS reader that uses the Neuronal Profiling BioApplication software module to identify the synapse measured by co-localisation of the pre-synaptic marker with the post-synaptic marker.
The Thermo Scientific Synaptogenesis kit has been validated in cellular imaging assays for reliable and reproducible results and it works on all HCS platforms.
In addition to HCS instruments, cells labelled by the kit reagents can be viewed and analysed by other fluorescence microscopes.
The kit also saves research time because it requires no optimisation of assay development.
