Harvard Apparatus offers 192-well DispoEquilibrium Dialyzers that provide methods to analyse up to 96 samples simultaneously on disposable dialysis plates in ligand-binding experiments.
The ready-to-use equilibrium dialysis plates are said to be convenient and cost-effective tools for ligand binding experiments including serum protein binding, protein-drug binding, protein-protein binding and protein-DNA binding assays.
Equilibrium dialysis is said to be the most preferred technique for molecular binding studies as it allows for the direct assays of molecular interactions at close to physiological conditions with minimal effects on equilibrium parameters.
It has also shown itself to be a valuable technique for sample preparation before candidate analysis by mass spectrometry.
Studies have shown enhanced target identification when equilibrium dialysis is used.
Each test well in the plate consists of two chambers separated by a regenerated cellulose membrane with a molecular weight cut off of either 5,000 or 10,000 Daltons.
Each chamber holds up to 300ul of sample or buffer.
To decrease the dialysis time, the plate needs to be rotated through 360-deg in a vertical position.
Harvard Apparatus can also supply a one- or two-plate rotator or an eight-plate rotator with temperature control.
The 96-well plate was designed with SBS footprint and well spacing to meet automation needs.
Other equilibrium dialysis products from Harvard Apparatus include the single-sample DispoEquilibrium dialyser for volumes up to 100ul, the re-usable PTFE Fast Micro-Equilibrium dialyzer for samples from 25ul to 1,500ul, and the Multi-Equilibrium Dialysis System for up to 20 samples with volumes from 200ul to 5ml.
