Thermo Fisher Scientific has launched a split-free, nano-flow UHPLC system that facilitates the dedicated separation of biomolecules, such as proteins and peptides, at ultra-high pressures.
The Thermo Scientific Easy-NLC 1000 is the newest addition to the Easy-NLC range of nano-LC products.
The instrument is easy to use and can seamlessly integrate with the complete range of Thermo Scientific mass spectrometry (MS) systems for instant, trouble-free operation.
The instrument will be displayed for the first time in the Thermo Scientific hospitality suite at the Hyatt Regency in Centennial Ballroom D during the 59th Annual ASMS Conference on Mass Spectrometry and Allied Topics, on 5-9 June in Denver.
The Thermo Scientific Easy-NLC 1000 uses shorter gradients on 50um UHPLC columns with 1.9um beads, delivering strong separation capability with razor-sharp peaks.
As a result of its advanced design, the Easy-NLC 1000 facilitates as much as 30 per cent more peptide and protein identifications by providing higher pressure ratings, along with independent and simultaneous variations of bead size and column length.
Increasing the number of identifications is a significant achievement for proteomic research and discovery purposes.
The Easy-NLC 1000 operates on the Thermo Scientific Xcalibur software platform, easily connecting and interfacing with the full suite of Thermo Scientific LC-MS systems.
The higher operating pressure range of the Easy-NLC 1000 significantly shortens analytical cycles and is designed to increase productivity by accelerating sample loading and column re-equilibrations.
Additionally, the split-free, nano-flow capability of the new system providesretention-time reproducibility to below 0.4 per cent RSD, even for very complex samples.
