Association for Cell Biology meeting in San Francisco, USA, will see the launch of seven new Elisa mutation detection kits for genotyping
Panomics,a developer of tools for rapid and easy analysis of cell signalling and transcription regulation, says it will be exhibiting seven significant new products for high throughput screening (HTS) at the 2003 Association for Cell Biology 43rd Annual Meeting and Exhibit in San Francisco, California, 12-17 December 2003.
Panomics's new SNPCapture Elisa mutation detection kits for genotyping employ a novel, patent pending technology in a hassle-free, high throughput Elisa platform. Everything you need in the kit is included.
No expensive equipment is required to start or read the assay.
Panomics says that, unlike other available methods, SNPCapture kits are 100% accurate.
The principle behind SNPCapture technology is marvellously simple.
The DNA sample of interest is amplified along with a reference of a known genotype using specially designed, biotinylated primers.
During PCR, a unique DNA structure known as a Holliday junction forms at the SNP site.
If the target and reference duplexes share the same genotype, the Holliday junction resolves into double-stranded DNA; if the genotype differs, the Holliday junction stabilises.
The next step is detecting the stabilised Holliday junctions.
The RuvA protein, which binds to Holliday junctions, is added along with streptavidin-HRP.
The mixture is then incubated in a 96-well microplate well treated with anti-RuvA antibodies.
The presence of Holliday junctions - indicative of a genotype difference - can then be visualised by a simple colour reaction.
There is no need to run gels.
This technology is 100% accurate, provides high throughput, simple, inexpensive and is an all-inclusive kit.
