With up to ten-fold higher signal-to-noise ratios than wild-type coelenterazines, reliable quantification of low levels of expression for reporter gene detection and Bret is achievable
Promega says that, following the launch of its Enduren live cell substrate, for the first time it is possible to measure Renilla luciferase luminescence for at least 24 hours after substrate addition.
The new substrate enables real-time live cell kinetic experiments over extended periods.
With up to ten-fold higher signal-to-noise ratios than wild-type coelenterazines, reliable quantification of low levels of expression for reporter gene detection and Bret is achievable.
Furthermore, because the Enduren substrate has been uniquely engineered with protected oxidation sites, substrate degradation and consequent background autoluminescence is minimised.
The extended signal duration also allows adaptation of assays to an automated, microplate format without the requirement for auto-injectors.
Consequently, sample throughput can be increased since there are no time-consuming per reagent injection steps.
The Enduren substrate is added directly to the culture medium, with no further reagent additions necessary when measuring luminescence.
This means that it is possible to perform repeat measurements using a single cell population.
It also allows multiplexing, using the same sample set and pairing with any lytic assay, thereby enabling more dynamic experiments to be performed.
