Transfection reagent, based on a lipid formulation, provides efficient transfection of siRNA into a wide range of eukaryotic cells with low cytotoxicity
Qiagen continues to expand its range of products and services for RNAi applications.
Transfection of siRNA is a critical step in gene silencing experiments.
RNAiFect transfection reagent, based on a lipid formulation, provides efficient transfection of siRNA into a wide range of eukaryotic cells.
RNAiFect reagent provides the valued combination of high transfection efficiency with low cytotoxicity.
Its low endotoxin levels and testing for absence of RNase activity ensure excellent and reproducible performance in the most demanding RNAi experiments.
It can be used for transfection of a wide range of cell types, including AGS, HEK-293, HeLaS3, Huh-7, Huvec, NIH-3T3, and PA317 cells.
RNAiFect reagent is provided in a ready-to-use form for easy transfection - simply add the reagent to the diluted siRNA, mix, incubate, and pipet the complexes on to the cells.
Transfection can be performed in the presence of serum, eliminating the need to remove the complexes from the cells.
Formation of transfection complexes occurs efficiently in complete growth medium.
In some cell lines, the silencing effect is significantly enhanced by replacing growth medium with buffer EC-R during complex formation.
RNAiFect reagent is also available in bulk for high-throughput experiments.
