Provides a library of ready-to-print oligos with the flexibility to spot oligos and detect signals using various printing and hybridisation techniques
Qiagen announces the release of the version 3.0 array-ready oligo set (Aros) for the rat genome.
The Rat Aros version 3.0 contains 26,962 70mer probes, representing 22,012 genes and 27,044 gene transcripts.
Qiagen uses state-of the-art methodology and proprietary software for probe design.
The Rat Aros version 3.0 design is based on Ensembl, a cutting-edge, public, open-source database and genome browser.
The Ensembl Rat 19.3b.2 database, based on the Rat Genome Sequencing Project, is the data source for the Rat Aros version 3.0.
Probe design allows detection of alternative splicing variants using common, partial common, or individual transcript oligos.
The Rat Aros comes with complete annotation provided by the Qiagen oligo microarray database (Omad) and Ensembl.
Omad offers comprehensive information about each oligo, and links to Ensembl, which allows access to further information, including gene ontology (GO), oligo chromosome coordinates, comparative genome analysis, and other functional annotation (InterPro, disease, and protein family).
The Rat Aros provides a library of ready-to-print oligos with the flexibility to spot oligos and detect signals using various printing and hybridisation techniques.
Each probe is melting-temperature normalised and includes a 5' amino linker to facilitate substrate attachment.
Each Aros is provided in 384-well plate format with 600pmol of product per well.
A control plate with 24 controls in 16 replicates is also included.
Aros sample sets, which include 96 optimised 70mers in 384-well plate format at 600pmol, are also available.