The kit is compatible with animal cells and easy-to-lyse tissues and provides purified RNA that is highly suited for sensitive downstream applications such as quantitative, real-time RT-PCR
The RNeasy Plus mini kit integrates fast and convenient RNA purification with effective elimination of genomic DNA contamination.
The kit is compatible with animal cells and easy-to-lyse tissues and provides purified RNA that is highly suited for sensitive downstream applications such as quantitative, real-time RT-PCR.
Genomic DNA contamination is effectively eliminated through the use of unique gDNA Eliminator spin columns in combination with an optimised lysis buffer.
Cell or tissue lysates are centrifuged through the spin columns, which rapidly and selectively remove genomic DNA from the lysates.
No lengthy DNase digestions are required.
High and reproducible yields of total RNA are achieved through the combination of buffer RLT Plus and RNeasy spin columns.
The buffer provides optimal lysis conditions, ensuring the release of all RNA in the sample.
RNeasy spin columns bind total RNA with high efficiency, allowing stringent washes to remove contaminants prior to elution.
Less than 25 minutes are required to purify total RNA and eliminate genomic DNA contamination.
The purified RNA is of high quality and ready to use in sensitive applications, such as real-time RT-PCR, and also in standard applications.
High Agilent RIN values can be obtained, indicating highly intact RNA.
